A Shocker in Berkeley

A Shocker in Berkeley

The Wichita State Shockers–truly a legend among college mascots.

The Rothfels lab was blessed with a visit from a proud shocker, the legendary Dr. James Beck, of Wichita State. “Shocker” — one who harvests wheat into shocks? Or, in this case, an animated shock itself:

 

 

 

 

 

James was here for a labwork blitz, refining a protocol for generating genome-scale data from herbarium specimens using double-digest RAD sequencing (ddRAD). Over the course of a week, he and Ingrid ground through two plates of samples, from beginning to end. Very excited to see how this works out!

This is what labwork looks like in the Rothfels lab. Personally, I think they’re having too much fun.
The delicate work of helping ethanol evaporate. Valuable use of time!!

In between pipetting and bead clean-ups, James squeezed in some spore counting from apomictic Myriopteris gracilis (32 spores/sporangium = apomict; 64 spores/sporangium = sexual). In a flurry of glycerol and microscopy, he was able to count his 600th specimen–look for the results soon in a journal near you.

27, 28, 29, …

James’ visit also provided us with an excuse to get outside and look for some lycophytes! Over the course of some happy tromping in Marin Co. we found both Isoetes howellii and Isoetes nuttallii, as well as a nice smattering of ferns.

Forrest working on his Isoetes nuttallii sampling.

 

 

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